Does Mushrooms Come Up on a Drug Test? What to Know
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Time to read 6 min
If you’re Googling “does mushrooms come up on a drug test,” you’re not alone. A lot of people wonder whether eating mushrooms—especially psychedelic ones like magic mushrooms (psilocybin)—can cause a failed drug screen.
Others just want to make sure their functional mushroom supplements (like lion’s mane, chaga, or reishi) are safe if they’re getting tested for work, sports, or legal reasons.
This blog will break down the difference, explain which mushrooms might show up, and answer whether you need to worry at all.
Wondering what "functional mushrooms" are? Checkout our blog, Types of Mushrooms: What Makes Functional Mushrooms Unique
The Essentials | TL;DR
Standard drug tests do not screen for psilocybin
Psilocybin clears quickly—usually within 24 hours
Functional mushrooms contain no psychoactive compounds
You cannot overdose on functional mushrooms
Psychedelics have both potential risks and powerful long-term benefits when used intentionally
Psychedelic mushrooms typically do not show up on standard drug tests. Unless you’re given a specialized test that specifically looks for psilocybin, it’s extremely unlikely they’ll be detected.
Functional mushrooms—like lion’s mane, chaga, or reishi—are completely safe, non-psychoactive, and will never cause a positive drug test.
Table of contents
Will Psychedelic Mushrooms Show Up on a Drug Test? How Long Does Psilocybin Stay in Your System? Functional Mushrooms Will Not Trigger Drug Tests. Can You Overdose on Mushrooms? Functional Mushrooms (Like Ours): No Psychedelic Mushrooms: Not Fatal, But Not Risk-Free Mushroom Side Effects: Short and Long-Term Functional Mushrooms: Very Low Risk Short-term Effects Long-term Effects Psychedelic Mushroom: Risks and Rewards Short-term Effects Long-Term Potential Benefits Risks (Context-Dependent) Final Thoughts: Know Your Mushrooms Frequently Asked Questions
Does Mushrooms Come Up on a Drug Test?
If you’re talking about psychedelic mushrooms and wondering does mushrooms come up on a drug test, the answer is: usually not.
Here’s why:
Psilocybin and psilocin (its active metabolite) are not part of common 5-panel or 10-panel drug screenings
These tests are designed to detect substances like THC, cocaine, opiates, amphetamines, and PCP
Psilocybin doesn’t chemically resemble these drug categories, so it doesn’t trigger a flag
That said, specialized drug tests do exist that can detect psilocybin or psilocin, but they are:
Rarely used outside of military, parole, or forensic settings
Expensive and not part of standard workplace or athletic screening
So unless you’re undergoing a very specific test, psychedelic mushrooms are unlikely to show up on a drug test.
Functional mushroom extracts like ours (that are NOT psychedelic) are completely safe and will never trigger a drug test.
How Long Does Psilocybin Stay in Your System?
Even though psychedelic mushrooms aren’t usually tested for, it’s still smart to know how long psilocybin (and its metabolite, psilocin) remains in your body.
Here’s the typical detection window:
Urine: up to 24 hours after ingestion (rarely tested)
Blood: detectable for 1–2 hours—but clears quickly
Hair: in theory, can remain for up to 90 days, but psilocybin hair testing is extremely uncommon
Psilocybin is metabolized and cleared fairly quickly, which is why even specialized drug tests have a short window to catch it. Unless you’re tested within a few hours of use—and specifically for psilocybin—it’s unlikely to be detected.
Functional Mushrooms Will Not Trigger Drug Tests.
Let’s be clear: if you’re taking functional mushrooms like lion’s mane, cordyceps, reishi, or chaga—you have absolutely nothing to worry about.
These mushrooms:
Do not contain psilocybin or any psychoactive compounds
Are considered completely legal dietary supplements
Will not show up on any type of drug test
At Hamilton’s, our extract powders are made from 100% fruiting body mushrooms—no mycelium fillers, no hidden additives, and no risk of triggering a test.
So if you’re using Mind Mix or Body Blend, you’re supporting focus, immunity, and recovery—without even touching the world of controlled substances.
Can You Overdose on Mushrooms?
It depends on the type of mushroom—but here’s the breakdown:
Functional Mushrooms (Like Ours): No
Mushrooms like lion’s mane, turkey tail, or reishi are incredibly safe when taken as supplements. There is no realistic risk of overdose with functional mushroom extract powders.
At worst, taking too much may cause:
Mild digestive discomfort
A loose stool or slight bloating
Very rarely, an allergic reaction
But even at high doses, you cannot overdose on functional mushrooms in the clinical or toxic sense.
Psychedelic Mushrooms: Not Fatal, But Not Risk-Free
Psychedelic mushrooms can absolutely be taken in excessive amounts, but they are not considered physically toxic or fatal.
Overconsumption may cause:
Panic attacks
Hallucination-related accidents
Psychological distress
Persistent anxiety in vulnerable users
So while you can take too many psychedelic mushrooms, it’s not an “overdose” in the classic medical sense.
Mushroom Side Effects: Short and Long-Term
When talking about mushroom side effects, it’s important to separate functional mushrooms (like lion’s mane and reishi) from psychedelic mushrooms (like psilocybin-containing species). Both have very different profiles—and in many cases, potential benefits.
Functional Mushrooms: Very Low Risk
For most people, functional mushrooms like lion’s mane, reishi, and chaga are well-tolerated.
Short-term Effects
Rare and typical mild:
Digestive discomfort (if taken in large amounts)
Temporary fatigue or alertness depending on the species
Occasional allergic reactions in sensitive individuals
Long-term Effects
Studies suggest functional mushrooms are safe for extended use—especially when consumed as properly extracted powders. There’s no evidence of long-term harm when used appropriately. Many users report improved energy, mood balance, and immune resilience with consistent use.
Learn more about the longterm benefits here:
Turkey Tail Mushroom: Immune Benefits of Functional Fungi
The Best Mushroom Supplements for Natural Energy Support
Psychedelic Mushroom: Risks and Rewards
Psilocybin-containing mushrooms have gained scientific recognition for their potential to improve mental health, particularly when used in guided or intentional settings.
Short-term Effects
Varies by dose and setting:
Altered perception of time and space
Emotional release, insight, or a sense of connectedness
Visual patterns, laughter, or euphoria
Possible nausea or anxiety at onset (especially with high doses or poor environment)
Long-Term Potential Benefits
Growing research suggests that psilocybin use may support:
Reduced symptoms of depression and anxiety
Increased emotional openness and resilience
Long-lasting shifts in perspective, meaning, and purpose
Risks (Context-Dependent)
- Distressing experiences or panic if taken in unsafe or unsupported settings
- Potential for psychological unease in people with underlying conditions
- In rare cases, lingering perceptual effects (HPPD)
TL;DR: functional mushrooms are safe and supportive for daily use, while psychedelic mushrooms hold powerful therapeutic potential when used responsibly and legally.
Curious About Psychedelics? Consider Lion’s Mane.
If you’re searching “does mushrooms come up on a drug test,” there’s a good chance you’re exploring psychedelic mushrooms—or at least thinking about it.
While Hamilton’s doesn’t sell or promote psilocybin, we do offer one of the most powerful legal mushrooms you can use to support the brain: lion’s mane.
This mushroom is a key component of the popular Stamets Stack—a protocol that combines:
Psilocybin (in places where it’s legal)
Lion’s Mane (to support neurogenesis and nerve growth)
Niacin (to improve absorption)
Our Lucid Lion's Mane contains both fruiting body lion’s mane extract and a premium mycelium extract rich in erinacines—the compound most associated with new brain cell growth.
So whether you’re experimenting, microdosing, or just looking for a cognitive edge, lion’s mane is a potent, legal way to support clarity, resilience, and long-term brain health.
👉 Learn more about Lucid Lion's Mane or check out How Lions Mane Mushroom Can Help Improve Your Memory
And of course—we only support legal, informed, and ethical use of psychedelic mushrooms.
Frequently Asked Questions
Do mushrooms show up in drug tests?
Standard drug tests do not screen for psilocybin (the psychoactive compound in magic mushrooms). Unless a specialized test is used—which is rare—psychedelic mushrooms will not show up.
How long can mushrooms stay in your system?
Psilocybin is metabolized quickly. It’s detectable in:
Urine: up to 24 hours
Blood: 1–2 hours
Hair: theoretically up to 90 days, but this test is almost never used
Will mushroom coffee show up on a drug test?
No. Most mushroom coffee is made with functional mushrooms like lion’s mane, chaga, or cordyceps. These do not contain psilocybin and will not trigger a positive drug test.
Hamilton Pevec
Hamilton believes that effective, affordable medicine is a fundamental right. Healing should never be hindered by cost. Driven by this belief, Hamilton left his career to dedicate his life to mushrooms. His journey is one of lifelong learning—mastering mushroom identification, cultivation, and understanding the profound physiological benefits of these natural “chemical factories.” Hamilton’s Mushrooms is dedicated to making pure, potent, science-backed mushroom extracts accessible to all.